| Name | Years at KU | Years in MLB | MLB Team(s) |
| Bob Allison | 1954 | 13 | Washington, AL (1958-60), Minnesota, AL (1961-70) |
| Ferrell Anderson | 1937-38 | 2 | Brooklyn, NL (1946), St. Louis, NL (1953) |
| Wes Benjamin | 2012-14 | 2 | Texas, AL (2020-21) |
| Jeff Berblinger | 1990-93 | 1 | St. Louis, NL (1997) |
| Brett Bochy | 2008-10 | 2 | San Francisco, NL (2014-15) |
| Herb Bradley | 1922-25 | 3 | Boston, AL (1927-29) |
| Clay Christiansen | 1977-79 | 1 | New York, AL (1984) |
| Don Dennis | 1961 | 2 | St. Louis, NL (1965-66) |
| Chuck Dobson | 1964 | 9 | Kansas City, AL (1966-67), Oakland, AL (1968-1971, 1973), California, AL (1974-75) |
| Robert Edmondson | 1898 | 2 | Washington, AL (1906, 1908) |
| Sam Freeman | 2008 | 9 | St. Louis, NL (2012-14), Texas, AL (2015), Milwaukee, NL (2016), Atlanta, NL (2017-18), Los Angeles, AL (2019), Washington, NL (2020) |
| Dale Gear | 1892-94 | 3 | Cleveland, AL (1896-97), Washington, AL (1901) |
| Tom Gorzelanny | 2002 | 12 | Pittsburgh, NL (2005-09), Chicago, NL (2009-10), Washington, NL (2011-12), Milwaukee, NL (2013-14), Detroit, AL (2015), Cleveland, AL (2016) |
| Harry Huston | 1902-06 | 1 | Philadelphia, NL (1906) |
| Skip James | 1970-71 | 2 | San Francisco, NL (1977-78) |
| Steve Jeltz | 1978-80 | 8 | Philadelphia, NL (1983-89), Kansas City, AL (1990) |
| Bill Kelso | Unknown | 4 | Los Angeles, AL (1964), California, AL (1966-67), Cincinnati, NL (1968) |
| Travis Metcalf | 2002-04 | 2 | Texas, AL (2007-08) |
| Larry Miller | 1957-58 | 3 | Los Angeles, NL (1964), New York, NL (1965-66) |
| Colton Murray | 2009-11 | 2 | Philadelphia, NL (2015-16) |
| John Nelson | 1998-2001 | 1 | St. Louis, NL (2006) |
| Ray Pierce | Unknown | 3 | Chicago, NL (1924), Philadelphia (1925-26) |
| Steve Renko | 1964-65 | 15 | Montreal, NL (1969-1976), Chicago, NL (1976-77), Chicago, AL (1977), Oakland, AL (1978), Boston, AL (1979-80), California, AL (1981-82), Kansas City, AL (1983) |
| Curt Schmidt | 1991-92 | 1 | Montreal, ML (1995) |
| Roger Slagle | 1974-76 | 1 | New York, AL (1979) |
| Scott Taylor | 1986-88 | 1 | Texas, AL (1995) |
| Les Walrond | 1996-98 | 3 | Kansas City, AL (2003), Chicago, NL (2006), Philadelphia, NL (2008) |
| Smoky Joe Wood | Unknown | 11 | Boston, AL (1908-1915), Cleveland, AL (1917, 1919-20) |
| Mike Zagurski | 2004-05 | 6 | Philadelphia, NL (2007, 2010-11), Arizona, NL (2012), Pittsburgh, NL (2013), New York, AL (2013), Milwaukee, NL (2018) |
